Combinatorial hydrogels with biochemical gradients for screening 3D cellular microenvironments.

Analysis of synthesized aggrecan showed a negative trend between RGD peptide concentration and aggrecan volume (Fig. 3a, b ).

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As observed with nuclear Sox9 fluorescence, there was also a positive trend between synthesized aggrecan and HAV peptide concentration (Fig. 3c, d ).

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Furthermore, curves at a fixed HAV concentration along decreasing RGD concentration showed an increasing trend (Fig. 6f ), with the highest aggrecan volume observed on the high HAV concentration curve with a low concentration of RGD (Fig. 6f ).

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Furthermore, the three curves feature a decreasing trend, with the lowest Sox9 value belonging to the high RGD concentration curve with lowest HAV concentration.

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