SAC1 degrades its lipid substrate PtdIns4<i>P</i> in the endoplasmic reticulum to maintain a steep chemical gradient with donor membranes.

Elife · 2018 · PMC5829913 · PMID 29461204

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closest p · 3.0× alpha

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did not reach statistical significancep=0.15not close (p > 0.1)

A fusion of FKBP to the N-terminus of SAC1 showed robust recruitment of the enzyme to ER-PM MCS within 1 min of rapamycin addition (see the inset graphs), and we detected a very subtle decline of PM PtdIns4 P as compared to the catalytically inactive C389S control, though this did not reach statistical significance (p=0.15, Tukey's multiple comparison test).

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