highly significantP < 0.001
When using multivariate regression analysis to adjust for the confounding factors maternal age, mode of birth, gestational age, birth weight and sex of the child, ATG4C assay 1 revealed a small but highly significant hypermethylation in both the ICSI ( P < 0.001) and IVF group ( P = 0.002), compared to controls (see Supplementary data, Table SII ).