4000 years of human dietary evolution in central Germany, from the first farmers to the first elites.

Distribution of δ 15 N and δ 13 C in the different age groups displays highly significant differences ( F (5, 405) = 8.21, p < 0.0001 and F (5, 405) = 5.06, p = 0.0002, respectively).

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A more detailed analysis based on the estimated interquartile ranges in each culture did not reach statistical significance ( F (1,8) = 2.02, p = 0.193 for δ 15 N and F(1,8) = 0.76, p = 0.410 for δ 13 C) ( S4 Fig ).

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To investigate a possible trend in the population variation with respect to the two stable isotopes, we determined the interquartile range within each culture and subjected the observed values to a meta regression with the period (numbered from 1 to 6) as covariate.

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We could observe increased δ 15 N values in infans I and an increasing trend from infans II to senile individuals. δ 13 C showed a decreasing trend.

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δ 13 C showed a decreasing trend.

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