A novel assay for the introduction of the vinyl ether double bond into plasmalogens using pyrene-labeled substrates.

While the reduction of disulfide bonds by TCEP did not have an effect, all three tested thiols strongly inhibited the desaturase reaction at 1 mM concentrations, with a clear trend toward strongest action of DTT, followed by comparable action of DTE and mercaptoethanol ( Fig. 3D ).

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