After 72 h in replete conditions (100% medium), the intracellular concentrations of methionine, choline, and betaine started to increase toward control cell concentrations, although these levels did not reach statistical significance compared to 168 h depleted cells (Fig. 1 b–d).
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Methyl-donor depletion of head and neck cancer cells in vitro establishes a less aggressive tumour cell phenotype.
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