With the exception of lactate, which accumulated over time, each measured component showed a trend toward additional consumption as culture length increased (observed ranges of the normalized media components: 0.49–1.35, 0.62–1.49, 0.49–1.79 at days 2, 4, and 6, respectively).
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Establishing quasi-steady state operations of microphysiological systems (MPS) using tissue-specific metabolic dependencies.
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