Similarly to when the spleen was removed in conjunction with FL engraftment, splenectomy 30 days prior to FL transfer gave rise to peritoneal and bone marrow B-1a cells, albeit at reduced frequencies, and lower serum IgM antibody levels than in the sham-operated mice, albeit this did not reach statistical significance ( p = 0.09) (Figures S3A–E in Supplementary Material).
1
0.0900
0.0900