Membrane binding, internalization, and sorting of alpha-synuclein in the cell.

First, we performed membrane biotinylation assays with the different mutants, and detected a clear trend in the amount of protein present in the biotinylated fractions that reflected the different membrane binding properties of the aSyn mutants (aSyn A30P and aSyn A11P/V70P) (Fig. 5a and b ).

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