Separating the effects of nucleotide and EB binding on microtubule structure.

Proc Natl Acad Sci U S A · 2018 · PMC6142192 · PMID 29915050

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a clear trendno p-value reported

By comparing the three nucleotide states (GMPCPP, GDP, and GTPγS) of MTs with and without bound EB3, there is a clear trend that, at least at the high concentrations used in the cryo-EM studies, EB3 introduces an additional left-handed lattice twist to whatever MT lattice it binds (even for 14-PF MTs).

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