Examination of the lipid disributions for individual months shows that starting from January, the nymphs had relatively high lipid reserves, which showed a trend to increase towards March, at which point most of the nymphs had lipid values higher than the median of all the nymphs collected during the two years, which was 14.9 μg (Figs 5 , 6 ).
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Spectrophotometric analysis of lipid used to examine the phenology of the tick Ixodes ricinus.
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