Comparing the H2 relaxin responses as a percentage of the forskolin response at 5 minutes showed a trend toward higher cAMP activity in PTX‐treated cells at higher concentrations of H2 relaxin, suggesting that PTX is inhibiting the Gα oB inhibition, but this was not significant ( P > 0.05) (Figure 8 C).
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Real-time examination of cAMP activity at relaxin family peptide receptors using a BRET-based biosensor.
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