Apicoplast BioID identified 56% (45/81) of the classified lumenal proteins and 33% (5/15) of the nonlumenal proteins ( Fig 3B ), suggesting that the GFP–BirA* bait used can label both lumenal and nonlumenal proteins but may have a preference for lumenal proteins (though this difference did not reach statistical significance).
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Integrative proteomics and bioinformatic prediction enable a high-confidence apicoplast proteome in malaria parasites.
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