High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons.

However, expression of Reln was consistently lower in samples recovered from cryostorage with an average fold change of 0.23 ± 0.03 compared to freshly dissected samples, although this did not reach statistical significance (multiplicity adjusted p = 0.086).

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We believe this protocol represents a highly significant improvement on past methods and makes neuronal cryopreservation a feasible and efficient alternative to fresh dissections.

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