difference was apparentP = 0.006
As far as amniocentesis is concerned, a quite recent matched case-control study (63 infants whose HBsAg-positive mothers had underwent the procedure and 198 newborns whose HBsAg-positive mothers had not underwent amniocentesis) found that HBV MTCT was more frequent among cases (6.35% vs 2.53%; P = 0.226); notably, the difference was apparent when maternal viral load was taken into account, especially above the threshold of 200000 IU/mL (50% vs 4.5%, P = 0.006)[ 84 ].