A crucial question to be asked is how the analysis results ought to be interpreted if they are contradictory, for instance, a situation where one rarefaction technique indicates an increasing or a decreasing trend but the other illustrates a flat trend that implies no changes in species richness.
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On species richness and rarefaction: size- and coverage-based techniques quantify different characteristics of richness change in biodiversity.
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In contrast, the cyan histogram for the coverage-based rarefaction measure indicates a negative trend in species richness (Fig. 2 , bottom panel), as does the observed richness.