In addition, a strong trend towards the NMN-treated old mice showing reduced freezing levels compared to the PBS-treated old mice ( p = 0.052) was found, with a major component of this trend being due to a large difference between groups observed during minute 3 ( p = 0.001).
The auditory cue test on day 3 revealed nonsignificant effects for the group variable and the group by time interaction, although there was a trend toward significance for the interaction effect ( p = 0.063), and there were trends towards differences in freezing levels between the CA1 Nampt KD and GFP CON mice at 3 min ( p = 0.049), 9 min ( p = 0.057), and 10 min ( p = 0.061).