Similarly, measurement of total circulating IgG antibody by ELISA assay in challenged WT, Tcrbd −/− , and SAP -ve B6 recipients of bm12 CD4 T cells revealed higher levels of IgG levels in WT group , when compared to the Tcrbd −/− and SAP -ve B6 recipient groups, but this did not reach statistical significance ( Fig. 5 d). 2.5 in vitro endothelial cell migration assays The response of culture bm12 endothelial cells to autoantibody was assessed by in vitro endothelial cell migration assay, using whole sera or column purified immunoglobulin isolated from recipient mice sera at different time points after transplantation.
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