Retinal Iba-1+ cell counts were decreased in the selumetinib RD group compared with the vehicle RDs, but this did not reach statistical significance (813.85 ± 140.19 cells/mm 2 selumetinib RD versus 1057.58 ± 65.99 cells/mm 2 control RD; P = 0.1469; Fig. 5 F).
In RD, we found pERK increased significantly in detached retina at day 7 and had a trend toward significance at day 3 compared to control retina ( P = 0.0025 day 7 and P = 0.0960 day 3); pERK accumulation was observed in Müller glia endfeet in the GCL and was very abundant in the inner nuclear layer (INL), co-localizing with Sox2 in the Müller glia nuclei with weaker levels in the cytoplasm.