Serine protease modulation of Dependence Receptors and EMT protein expression.

Spot chemotaxis assay In the spot chemotaxis assay using netrin-1 (1000 ng/ml) as the chemoattractant, the resting rate of migration of transfected cells was significantly greater than that of naïve cells, but the stimulant effect of netrin was less than in normal cells and did not reach statistical significance ( Figure 3H ).

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This trend became highly significant during incubation in 1% serum medium which induced a clear baseline expression of vimentin ( Figure 2K ) which was further increased by chymotrypsin (300 nM) and the low (30 nM) concentration of subtilisin.

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