Investigation of cell culture conditions for optimal foot-and-mouth disease virus production.

Calculations of the cell-specific glucose uptake rates revealed a positive trend of an increased glucose consumption with increased glucose availability when infected with FMDV serotype A that was not evident for cells infected with serotype O (see Additional file 3 : Table S3).

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The calculation of the cell-specific glucose and glutamine uptake as well as lactate production revealed an increasing trend for all three metabolites between a concentration of 2–10 mM NH 4 Cl in the media for cells infected with the FMDV A/IRN/8/2015 isolate, while no such trend was evident for cells infected with FMDV O/SAU/18/2015 (see Additional file 3 : Table S5).

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The comparison of the cell-specific uptake rates showed a negative trend for the mock-infected cells and cells infected in 30% fresh media with reduced glucose consumption at higher cell densities, whereas cells infected in 100% fresh media increased their glucose uptake (see Additional file 3 : Table S6).

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