Regional Differences in the Contributions of TNF Reverse and Forward Signaling to the Establishment of Sympathetic Innervation.

An increase in sympathetic innervation was also observed in Tnf −/− mice in whole mount preparations, although this did not reach statistical significance.

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Using a stereological method to estimate the extent of the growth and branching of sympathetic fibers within these tissues, we found highly significant decreases in Tnf −/− mice compared with Tnf +/+ littermates and Tnfrsf1a −/− mice compared with Tnfrsf1a +/+ littermates (Fig. 2 ).

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