approached significancep = 0.0545
The relative amount of TFAM-bound mtDNA, determined by qPCR ( Figure 3 B), was significantly smaller at Ori-L and DR1 regions ( p = 0.0434 and p = 0.0117, respectively), and approached significance at the D-loop ( p = 0.0545) in 32-month-old rats in comparison with younger counterparts.