Expression of Nanog, Sox2 and Oct4 by qRT-PCR analysis showed a trend of increased expression of these genes in MSCs plated on soft substrates when compared to the stiff substrate (Fig. 3F ), in line with what was already described for fibroblasts 41 .
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Soft culture substrates favor stem-like cellular phenotype and facilitate reprogramming of human mesenchymal stem/stromal cells (hMSCs) through mechanotransduction.
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