While the difference between wild-type and CspC G457R spores was statistically significant for all taurocholate concentrations tested, CspC R456G spores exhibited statistically significant increases in germination for the 0.5% (10 mM) taurocholate concentration, although it approached significance at the lowest taurocholate concentration tested (0.125%, ~2 mM).
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The CspC pseudoprotease regulates germination of Clostridioides difficile spores in response to multiple environmental signals.
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