Protein phosphatases regulate growth, development, cellulases and secondary metabolism in Trichoderma reesei.

In contrast, Δ rgb1 /TR_120545, Δ pph5 /TR_21256, Δ pph5-2 /TR_124001, Δ pty4 /TR_23417 and Δ pty5 /TR_53525 showed decreased specific cellulase activity and in Δ sit4a /TR_35316 we found a negative trend (Fig. 4A ).

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We found clearly increased specific cellulase activity compared to wildtype for Δ rgc1 /TR_123502 and a positive trend for Δ dsp1 /TR_80292 (Fig. 4A ). Δ rgc1 /TR_123502 exhibited a defect in hyphal growth (as reflected in achieved colony diameter) in light and darkness only when growing on cellulose, but not on malt extract (Fig. 2 ), indicating more efficient cellulase production at the expense of biomass formation.

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