approached significancep = 0.07
The reduction in the threshold voltage did not change significantly by increasing the interval Δ from 0.5 to 1 ms (1 ms wide pulses, p = 0.5, repeated measures ANOVA), but the difference approached significance for comparison between Δ = 1 ms and Δ = 5 ms intervals ( w = 5 ms pulses, p = 0.07, repeated measures ANOVA).