a significant trendP <0.001
Linear mixed-effects model analyses Salivary Fel d1 levels in relation to time and/or experimental factors The first saliva collection was performed in the morning, and there was a significant trend in both group 1 (β = −1.08, P <0.001) and group 2 (β = −1.76, P <0.001) for higher Fel d1 levels in the morning than in the afternoon ( Figure 2a,b ).