Consistent with this hypothesis, the expression of Nodal and GDF3 (a Nodal target) in the differentiating hPSC lines showed a strong trend indicative of their up-regulation linked with the induction of gastrulation-associated genes and their absence linked with the induction of preneurulation-associated genes ( Fig 3Ci ).
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High-throughput micropatterning platform reveals Nodal-dependent bisection of peri-gastrulation-associated versus preneurulation-associated fate patterning.
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