<i>Drosophila</i> PSI controls circadian period and the phase of circadian behavior under temperature cycle via <i>tim</i> splicing.

Importantly PSI came out of a screen of over 600 RNAi lines and was one of the rare genes to show a short period phenotype when downregulated, so it is clear that a 1.5 hour period shortening compared to driver control is highly significant, and specific.

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Knockdown of Psi in BG-LUC flies resulted in a similar trend, although differences did not reach statistical significance ( Figure 2—figure supplement 2C and D ).

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