Whatever the iron overload protocol, the induction of colonies took over 12 weeks of repeated cycles of cell culture on soft agar, and the number of colonies was quite significant at 20 weeks of culture (over 120 colonies of 25,000 cells) (Fig. 7d ).
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Iron chelation by curcumin suppresses both curcumin-induced autophagy and cell death together with iron overload neoplastic transformation.
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