Barely Significant
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A step-by-step protocol for isolation of murine nucleus pulposus cells.

JOR Spine · 2019 · PMC6920701 · PMID 31891122

Europe PMCDOI
1
hedged sentence
0.0600
closest p · 1.2× alpha
0.0600
boldest claim

The sentences

approaching significanceP = .06so close (0.05 < p ≤ 0.1)
None of the trends (linear, quadratic, or cubic) were significant (alpha = .05), though Figure 6 D suggests a positive linear increase in gene expression over time with a t value approaching significance (t = 1.9, P = .06, eta‐square = 0.94). 4 DISCUSSION The method described herein allows multiple and reliable qPCR analyses from lumbar and thoracic mouse IVD compartments and can circumvent the need for pooling IVDs from different spine regions or mice in nonsurgical models.

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