moderately significantp < 1 × 10 −5
The next 2 genes (low density lipoprotein receptor ( LDLR ) and tissue inhibitor of metalloproteinases metallopeptidase inhibitor 1 ( TIMP1 ) showed moderately significant upregulation (p < 1 × 10 −5 ) in gene expression between D20+ TGF-β and D20 samples, as detected by RNASeq analysis and a significant upregulation at D20+ TGF-β vs D20 by real-time PCR (Fig. 4H,I ).