Expression of Pax2, a progenitor cell-related transcription factor, and Oct4, a stem cell marker, by qRT-PCR analysis showed a trend of increased expression in RPCs plated on soft substrates when compared to stiffer substrates ( Figure 5 c), in line with what was already described for mesenchymal stem cells (MSCs) and fibroblasts [ 43 , 44 ], thus suggesting that short-term culture on a soft environment maintains RPCs in a more undifferentiated state.
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Substrate Stiffness Modulates Renal Progenitor Cell Properties via a ROCK-Mediated Mechanotransduction Mechanism.
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