Thermostabilization of VPR, a kinetically stable cold adapted subtilase, via multiple proline substitutions into surface loops.

All single proline variants showed a trend of higher λ max values (Table 2 ) indicating higher polarity around one or more of the Trp residues.

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At these temperatures accessibility to fluorophores did not seem so be affected to any extent as Stern-Volmer constants showed just a marginal trend of higher Stern-Volmer constants, often under one standard deviation (Supplementary Table 3 ).

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