High-resolution imaging mass spectrometry combined with transcriptomic analysis identified a link between fatty acid composition of phosphatidylinositols and the immune checkpoint pathway at the primary tumour site of breast cancer.

Histological PI(18:0/20:3) accumulation was observed more frequently in ER-positive/PgR-negative/human epidermal growth factor receptor 2 (Her2)-negative and triple-negative subtypes, although the difference did not reach statistical significance (Table 1 ).

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