However, an important shortcoming of this technique in insects is the mechanical damage during the injection, which is quite significant when targeting embryos and neonatal larvae and pupae (Scott et al. 2013 ; Yu et al. 2013 ).
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However, an important shortcoming of this technique in insects is the mechanical damage during the injection, which is quite significant when targeting embryos and neonatal larvae and pupae (Scott et al. 2013 ; Yu et al. 2013 ).