clearly significantp = 0.005
In this context, the mScarlet-based biosensor showed minimal response (0.3 ± 0.2%, n = 11), whereas the stagRFP counterpart showed a clearly significant FRET response (3.3 ± 0.4%, n = 8, p = 0.005, t = 3.642, df = 9) (Fig. 3a ).