Development and validation of a portable, point-of-care canine distemper virus qPCR test.

In heart, spleen, lung and brain, RNA viral copy number recovery was lower (0.5–2 logs) with the Biomeme M1 Sample Prep Kit TM , but did not reach statistical significance, and was comparable in other tissues that had similar viral recovery such as liver and muscle when normalized to total viral RNA recovered in the RNA extract ( Fig 5C ).

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