DNA content had a negative trend over time for the high cell density group, as suggested by the lower DNA content at day 14 compared to day 3 ( Figure 4 ).
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Primary Human Osteoblasts Cultured in a 3D Microenvironment Create a Unique Representative Model of Their Differentiation Into Osteocytes.
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Moreover, cells from the donor 1 showed a significantly higher ALP activity by day 7 followed by a decreasing trend ( Figure 5A ).