While control and the P[syt D-E ] mutant both showed a trend toward an increase in release events during the 20–300 ms asynchronous time window compared to prestim time window, these trends were not statistically significant ( Fig 4D , Table 1 , 25% increase for control, p = 0.11, t(84) = 1.62 and 11% increase for P[syt D-E ] , p = 0.52, t(64) = 0.65, paired t-tests).
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The role of the C2A domain of synaptotagmin 1 in asynchronous neurotransmitter release.
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