The estimated library size (a measure of the complexity captured by the RNA-Seq library) showed a trend towards correlating with detectability (Online Resource Fig. 1d), but had levels in both groups that were above those typically considered to cause general problems in fusion detection (< 30 million; authors’ unpublished observations).
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An optimized workflow to improve reliability of detection of KIAA1549:BRAF fusions from RNA sequencing data.
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