In SP1 msn2Δmsn4Δsko1Δhot1Δ , induction of RTC3 mRNA and RTC3-LacZ were low, but still quite significant (mRNA induced by 9-fold, compared to 50-fold in wild type cells; β-galactosidase reached 20 units compared to 100 in wt) ( Fig 1C and 1D ).
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Hog1-induced transcription of RTC3 and HSP12 is robust and occurs in cells lacking Msn2, Msn4, Hot1 and Sko1.
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