Effects of Continuous Nitrogen Fertilizer Application on the Diversity and Composition of Rhizosphere Soil Bacteria.

Among the other prominent bacterial phyla, we found that the average relative richness of Patescibacteria , Bacteroidetes , and Actinobacteria showed a trend of gradual increase with increased N fertilizer inputs during the first year of the present study, and Patescibacteria and Actinobacteria showed significant positive correlations with soil NO 3 – .

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The average relative richness of Actinobacteria , Bacteroidetes, and Patescibacteria gradually decreased with a prolongation of the treatment period ( Figure 4 ), with those of Bacteroidetes and Patescibacteria decreasing significantly and the decreasing trend observed for Bacteroidetes reaching an extremely significant level under continuous treatment with N 180 .

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