NS5-V372A and NS5-H386Y variations are responsible for differences in interferon α/β induction and co-contribute to the replication advantage of Japanese encephalitis virus genotype I over genotype III in ducklings.

Comparison of the levels of IFN-α and β production among groups of JEV-inoculated ducklings revealed that the ducklings inoculated with rGI/V372A-H386Y and rGIII/A372V-Y386H showed a trend in IFN-α and β levels opposite from those in ducklings inoculated with the respective parental viruses. rGI/V372A-H386Y with a Val to Ala substitution at NS5-372 plus a His to Tyr substitution at NS5-386 induced significantly higher levels of IFN-α and β production than the parental rGI, whereas the rGIII/A372V-Y386H with an Ala to Val substitution at NS5-372 plus a Tyr to His substitution at NS5-386 elicited clearly lower levels of IFN-α and β production than the parental rGIII ( Fig 10A and 10B ).

also in 53,322 other papers