After the addition of Hep+ protein fraction in the higher concentrations (250 and 500 μg/mL), anti-phosphotyrosine staining of spermatozoa demonstrated a positive trend in the groups with no and low signal.
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Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation.
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The sentences
Interestingly, in the case of adding the Hep+ SP protein to a 500 μg/mL concentration, there was a negative trend on sperm viability after thawing.