<i>setd2</i> knockout zebrafish is viable and fertile: differential and developmental stress-related requirements for Setd2 and histone H3K36 trimethylation in different vertebrate animals.

Furthermore, immunoblot analysis of H3K36me3 levels in the setd2 mutant embryos also showed a trend toward decrease of H3K36me3 in the homozygous embryos that carried maternal setd2 mRNA and protein, and this decrease was much slower than the setd2 mRNA degradation (Fig. 5e ).

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