Importantly, the osteoblast transcription factor Sp7 , encoding Osx, and the late-osteoblast/osteocyte maker Sost , encoding Sclerostin, were significantly decreased in tumor-bearing bones from Wls ΔOB -OS mice, while the decrease in Bglap mRNA, encoding the mature osteoblast marker Osteocalcin, did not reach statistical significance (Fig. 2c ). qPCR analyses in primary OS cells and Cre immunohistochemistry (IHC) used as a reporter for Sp7/ Osx expression by comparing Wls HET -OS and Wls ΔOB -OS tumors, confirmed reduced transcription of Sp7 , when Wls is inactivated (Supplementary information, S2g–i ).
1
—
—