How the O<sub>2</sub>-dependent Mg-protoporphyrin monomethyl ester cyclase forms the fifth ring of chlorophylls.

Test assays monitored the spectral changes and found a clear trend of decreased absorbance maxima of the substrate at 422, 552 and 592 nm, accompanied by increasing absorbance maxima for the product at 446, 586 and 634 nm ( Fig. 3a ).

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