Addition of mtSSB in increasing molar excess (50-fold and more) showed a trend towards a modest stimulatory effect (∼50%) on the activity of RNase H1, based on densitometry (see Fig. 6B ), although this was abolished when the assay was conducted in the presence of ssDNA ( Fig. 6B ).
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A second hybrid-binding domain modulates the activity of Drosophila ribonuclease H1.
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